Virology / Serology - Syphilis
- DHHS Serology Form #3445 (PDF, 385 KB) for confirmatory testing
- DHHS Syphilis Screening Serology Form #3446 (PDF, 185 KB) for TRUST screening
- Packing Instructions Using Outer Baggie (PDF, 4.6 MB)
- Lab Test Results Lookup
Syphilis, a disease caused by infection with the bacterium Treponema pallidum, can be readily diagnosed by serologic methods. Serologic assays used to screen patients for syphilis are nontreponemal tests. The nontreponemal test performed in this laboratory is the Toludine Red Unheated Serum Test (TRUST). Confirmation of reactive screening test results (TRUST) is obtained through the use of specific treponemal tests for syphilis. The TREP-SURE EIA, which detects both IgM and IgG antibodies to T. pallidum, is performed in this laboratory to confirm syphilis screening test results when appropriate.
Specimen Acceptance Policy
The nontreponemal test for syphilis (TRUST) performed on serum is available only to local health departments and state-operated health facilities. The specific treponemal test for syphilis (TREP-SURE EIA) is available to all health care providers but is not designed to be a screening procedure and thus is only performed when required for proper patient management.
Submitted specimens must be accompanied by a fully completed DHHS Serology Form #3445 (PDF, 385 KB) for confirmatory TREP-SURE EIA testing or DHHS Syphilis Screening Serology Form #3446 (PDF, 185 KB) for TRUST screening. Failure to supply the requested patient information may result in significantly delayed specimen testing or compromised test results. Only serum may be submitted for primary serologic syphilis testing. Specimens submitted for diagnostic testing not labeled with correct patient identification information will not be tested. Minimal patient specimen identification includes full first and last name and either SSN or date of birth. Specimens which, for any reason, are deemed unsuitable or inappropriate for diagnostic testing will not be tested and will be discarded. Specimens received without a test requisition will be properly stored for ten days pending verbal and/or written notification of the submitter. Unless a test requisition is received, the specimen will be discarded at the end of the holding period.
All screening tests performed in this laboratory which are determined to be reactive will be confirmed by the TREP-SURE EIA, unless a previous positive TREP-SURE or other confirmatory test result is on file at the laboratory. In those cases, only the screening test results will be reported.
A request to this laboratory for a TREP-SURE EIA test must be accompanied by a quantitative screening test result, i.e., the submitter must provide a titer. This request will yield only a qualitative TREP-SURE EIA result without performing a screening test. If a previous positive TREP-SURE or other confirmatory test result is on file at the laboratory, no testing will be performed.
For the purposes of evaluating patients suspected of having late syphilis, the TREP-SURE EIA will be performed in this laboratory on serum regardless of the screening test result. Under these circumstances, the submitter must specifically request a TREP-SURE EIA, state the quantitative screening test result/titer, and indicate that late syphilis is suspected.
To collect ≥ 2 mL of serum, draw ≥ 5 mL of blood into a red top vacuum tube, serum separator tube, or equivalent and let stand for ≥ 30 minutes to ensure complete clotting of the blood. Centrifuge the sample for 5-10 minutes at ≥ 1,000 x G. Transfer serum to a plastic screw-capped vial. Clearly label each vial of serum with the patient’s full first and last name and either SSN or date of birth. Grossly hemolyzed, icteric, or lipemic serum is unacceptable for syphilis serologic assays. For TRUST screening, complete DHHS Syphilis Screening Serology Form #3446 (PDF, 185 KB); for the TREP-SURE EIA test, complete DHHS Serology Form #3445 (PDF, 385 KB).
Properly identified vials of patient sera along with the completed submission forms should be sent in white colored specimen mailers labeled SYPHILIS SEROLOGY. For detailed shipping instructions using the double mailers, see Packing Instructions Using Outer Baggie (PDF, 4.6 MB). Ship at ambient temperature by the State Courier or U.S. Mail.
Serum transport tubes and white colored specimen mailers are available through the NCSLPH online supply ordering system at this website. The DHHS Syphilis Screening Serology Form #3446 (PDF, 185 KB) for TRUST screening and DHHS Serology Form #3445 (PDF, 385 KB) for TREP-SURE EIA tests (PDF, 385 KB) may be downloaded and printed.
Results of nontreponemal tests for syphilis (TRUST) performed on serum are available within two working days after receipt of the specimen. Treponemal specific tests (TREP-SURE EIA) performed on serum are available within three working days after receipt of the specimen. These results can be accessed on Lab Test Results Lookup.
Patients with primary syphilis may have a nonreactive TRUST and/or TREP-SURE EIA when first seen; however, these tests will usually become reactive soon thereafter. Most patients treated for primary syphilis have a reversion of nontreponemal tests to nonreactive within 2-3 years. The TREP-SURE EIA will usually remain reactive after treatment. Nonreactive serologic tests and normal clinical evaluations do not exclude incubating syphilis.
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